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The first portion was directly homogenized in ice-cold 10 mM phosphate buffer (pH 7.4) to prepare a 10% (w/v) homogenate, which was centrifuged at 5000 rpm for 10 min at 4 C

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Tirzepatide may be considered for adults who: struggle with losing body fat have weight-related health concerns experience insulin resistance gain weight easily despite lifestyle changes want medical support for sustainable weight loss Many men are particularly concerned about losing muscle mass during weight loss